Are genetically modified crops more harmful than beneficial to the Research Paper

Are genetically modified crops more harmful than beneficial to the environment - Research Paper Example It reviews scientific literature to investigate whether the genetically engineered crops are more harmful or more beneficial to the environment. Reports on scientific research and analyses have offered varied opinions on the impacts of genetically modified crops on the environment. A research initiative by Powel et al on the effects of genetically modified crops on their immediate soil identifies temporary effects on lives around the crops. The effect lasts for less than a year, especially when the crops are grown only once in an area. The crops are also associated with lowering the rate of organic compounds’ decomposition in soils for retention of primary nutrients in the soils to ensure a stable food chain that begins with plants. This therefore identifies advantages of genetic modification towards food security in an ecosystem (Powel et al, p. 394). Research by Brookes and Barfoot on the environmental effects of genetically modified crops also identifies significant benefits of the engineering. According to their research results, application of genetically modified crops that are resistant to weeds and insects have reduced the general trend in application of chemical based herbicides and pesticides to lower environmental pollution due to the involved chemicals. This has in effect promised a reduction of green house gas emission to environmental safety (Brookes and Barfoot, p. 193). The effect of the genetically modified crops’ ability to kill insects and weeds that threatens the crops is also a source of the technology’s harmful impacts on the environment. The crops for example extend their potential and kills insects and weeds that do not affect them. As a result, the genetic engineering leads to unnecessary destruction of abiotic factors. This harm is more significant if it affects insects or weeds with environmental value. The toxicity of the crops is similarly

Where Have You Gone Essay Example for Free

Where Have You Gone Essay Often in life, it is the choices we make that shape our identity. Our behaviors, and actions determine the outcome; ultimately becoming a part of our character. In the poem â€Å"Where Have You Gone? † by Mari Evans the author reveals the consequences of a tragic ending to an overly reliant relationship. Evans suggests that when in a relationship, often one individual chooses to rely too much on the other without realizing; building a heavy dependency, causing the loss of their own identity. Like Evans poem, I personally had to learn the consequences of my decisions which led to detrimental effects between my relationship with my sister. Through Evans illuminating poem we are able to assess the devastating effects of a poor woman who has been abandoned by her lover. Evans choice to use the title â€Å"Where have you gone? † conveys her sense of loss and confusion. The entire poem centers over this nagging question. The repetition of the same inquiry amplifies the growing bewilderment and confusion inside her, tearing her apart. Initially, we are led to believe we are reading an ordinary love poem with the reference of a woman who misses his â€Å"confident walk with [his] crooked smile† until Evans reveals him leaving with â€Å"the rent money in one pocket and [her] heart in another † This drastically changes the sense of superficiality in a relationship. For the woman this is not just heartbreak, but she has lost her sense of survival. It becomes apparent she is grieving when Evans states â€Å"with you went the sun. With the absence of sunlight, it leaves her in the darkness longing for his return. For his return will relieve her of distress and mourning. It is evident that she lost her sense of identity through her dependencies on him emotionally and financially. When I was a child, I looked up to my oldest sister. I wanted to look just like her. After entering junior high school, I made a new group of friends and changed the way I dressed and acted. I had begun copying my sister relentlessly. I made it my priority to sneak into her room and â€Å"borrow† her clothes. My decision to disrespect my sister’s belongings led to frequent arguments, and physical brawls. She began distancing herself from me and our once strong relationship vanished. Through this phase I lost the older sister I grew up with because of my obsession with merely wanting to fit in at school. Consequently, I became extremely lost as to who I was, unable to remember the person prior; leading me to live a life that was not mine. Eventually, I realized this contributed to the shattered bonds with my sister, and even resulted in losing my own identity. My experience suggests often individuals can become so caught up with others we lose sight of who we actually are. Mari Evans poem â€Å"Where Have You Gone? † is the after effects of having been abandoned by her lover. We witness the devastating effects of a woman who has been cripplingly reliant on another person. Sadly, situations like these happen when individuals choose to invest themselves too deeply into a relationship which causes an individual to lose their sense of self.

Diary Of Anne Frank :: essays research papers

The story Diary of Anne Frank was a very interesting book which showed the ways a group of Jewish people during the 1940's went about trying to conceal their identity and themselves. This story was a true story taken from a diary of a young girl during the incident. This was made into a play during 1955. This was praised as Frances Goodrich's and her husband Albert Hackett's most famous work as it was performed.The play was started in November of 1945. As Mr. Frank began to read the diary, it flashed back to July 1942 in an attic in Amsterdam because this was where the people were hiding and represented the type of place that Jews all over were living. The most important part of the play were the people who were acted out. They gave the play a sense of flavor and realism. Anne, a young German girl was particularly amusing because of the scuffles she and everyone around her seemed to have. Anne's Mother was a woman who was more traditional than anything else and wanted Anne to be more like a lady. One such person was Margot. As Anne's sister, she was very nice and didn't speak out and was very proper. The Frank's weren't the only ones in this attic, there were other people such as the Van Daans. Mr. Frank let them stay because they needed a place to hide and since they had helped him out so much in the past by actually teaching Mr. Frank German, he felt it was the least he could do. The Van Daans had a son which Anne later became interested in. Peter was the only person who Anne could understand and knew that Anne could understand him. They could both talk to each other freely when they were together. Dussel soon joined the group. He was only supposed to be up in the attic for a short time, but he ended up staying till the end. He had to leave his Dentistry to hide out from the Germans. These people would not of lasted too long without the help of Miep Gies and Mr. Kraler who gave them the necessities they needed to survive up in the attic for so long. All these people gave their own personality and views to add new dimensions to the play and make it more enjoyable.

Organizational Changes

In the past organizations have been faced with problems of change due to the introduction of new information technologies as the internet .Organizational change occurs when an organization desires to change its overall strategies for achieving its goals, when it wants to add or remove some sections of the organization and when it wants to change its way of operating its business.The leaders and managers are given the responsibility of implementing change in the organizations, but there have been cases where some have failed to carry out their activities because of their own selfish interests, lack of knowledge or ignorance on their part, although some leaders have carried out their activities effectively. It is difficult to implement changes in the organizations but with the good structured programs the organization can effectively implement its changes.In times of change people in organization rarely accept change because it will affect them in one way or another, thus a manager one needs to know how people are empowered in their organization so that they can utilize the skills of all employees in the organization.   Ã‚  People resist change because of individual fears, denial and anger the organization should accept the weaknesses of the workers and they should counsel them since it is at this particular time they require help so that they can accept themselves and assist them to perform as required. In cases where there are the employees are resisting changes at all circumstances they should be given other duties to perform.In the case of politics and prose bookstore in Washington D.C of Barbara Meade and Carla Cohen they owed the business but they decided to hire an independent manager known as Danny Gainsburg to run the business. The management failed to recognize the fact that employees who had worked in the organization were supposed to be empowered so that they could carry out the task of running the management but the management employed a different person this led to conflicts.The management should have empowered its own employees in to the organization since they had acquired  the necessary skill to run the business.   With the appointment of Mr. Danny Gainsburg as an entrepreneur in to the organization was a big threat to the organization since he had his own way of running the organization since he was not previously employed in the organization.When employees are empowered they are able to accomplish the tasks that they are assigned to do since they are given the powers and authority to do their tasks as they have been asked to do and this gives them the motivation to pursue their jobs effectively.Another factor that would   lead to the process of implementing the changes of the business the employees are   practices such as flexible job assignment in terms jobs rotations or flexible schedules this provides employees with a better overview of the organization and gives them the opportunity to improve and training w ould give employees a better understanding of the techniques used and potentially increase their capacity to deal with problems incentive pays such as line incentives, power sharing and bonuses would be used to reward the employees.For change to be made effective the top management must support the change process they must provide workshop, extra time, resources, and training to the employees so as to enable change to be implemented effectively in the organization. The top management must also train, coach, and manage the process they can accomplish this by re-arranging workloads, and allowing other workers to take part in the change process. The workers must be motivated by giving them staff leaves, overtime pays, and they must be encouraged to view work so that they can effectively achieve their goals.The organization must create a conducive environment for the workers so that they can accomplish their tasks these includes allowing them to correct their mistakes and punishing thos e who need to be punished since they have been known to resist change. The organizations needs to evaluate update and periodically improve its policies, procedures, plans, communication methods, new technologies, values, performance appraisals so that it can conform to what its expected to do and this can result in better results for the organization. http://www.managementhelp.org/misc/reqrs-for successful-change.pdf  Ms Meade and Ms Cohen should have communicated the changes of organization and this would have ensured that the employees were made aware of their tasks in the organization thus be able to achieve the objectives of the organization and the task of implementing the changes of the organization.At the time when the management informed the staff about the appointment of Mr. Gains burg in to the organization the appointment was appreciated because the employees were prepared to receive him although other people did not accept him since he didn’t have the necessary experience of carrying his activities. For Gainsburg to have learnt how to deal with the problems of the organization, he set standards that would ensure that he interacted properly with the employees, had an understanding of the duties and roles of the employees and this eliminated chances of getting a negative response from them.Ms Meade and Ms Cohen move to sell the organization without the employees knowledge was a wrong move since the employees should have been informed about the move so that they could prepare themselves adequately for the change since this would mean that they would lose jobs since a new management would bring in new employees in the organization.In course of doing business the organization realized high sales from its competitor the Barnes and noble Inc and borders group inc they said their success was attributed from having their staff being motivated, having the staff with the right skills and knowledge of doing their business and the employees being charg ed with the responsibility of handling all matters of the organization and this factors made the employees work effectively. Ms Meade and Ms Cohen should have motivated, chosen the right personnel in to the organization so that they would carry out their activities effectively and this would have boosted the performance of the company tremendously.The management decision to employ Mr. Gainsburg as a partner in to the organization was unexpected because he spend most of his time with owners of the business hence he acquired the knowledge of how the business was being run thus he became the manager of the organization. Under Mr. Gainsburg leaderships in these bookstores he brought changes since he had acquired the experience to run the business, he ensured changes were implemented effectively and he informed the employees about the all that was to be done. The duties and roles of the employees were defined thus he was able to carry out his task effectively.The other reason why Mr. Gai nsburg could have succeeded in running the business is that he had employees who worked as a team and did their work effectively and this lead to the businesses success.  He had personal attributes that lead to the success of the business such as he was ready to take the risk of taking up a business that was not performing; he was humble since he took time to learn the business. He was aggressive as he listened to the criticism of others and he accepted new ideas of operating the business and thus was able to take the opportunities that the business had this led to the successful implementations of the organizationMr. Danny Gains burg learnt   ideas that enabled him   achieve its objectives these were: he established the analyzes of competition and identified the problem of the organization and he led the team to change, he created a vision for the organization which acted as a guide on how the business was to be led, he communicated the vision, strategies and the expected beh avior of the employees towards the job, he identified people who could implement change and he ensured that the changes became part of the organizational culture for long-term transformation and growth.The success of the organizations performance was as a result of the new management being given the authority to carry out its activities independently with no interference of the other departments, the management gave the employees the rights of doing their business and also trained the staff on the right mechanisms of carrying out the business and a better environment for the business was created which enhanced quality results for the organization.In order to improve productivity in to the organizations employees must benefit financially from their contribution. This means that the employees must be motivated so that they can produce good results for the organization; they must be given incentives as good pay job security, job flexibility and leisure periods.   Ã‚  The members of t he organization should be enlightened on the need for change because people resist changes in the organization because of fear of the unknown, the inadequacy to deal with change and they fear the effect that change will have on their jobs.For the organization to successful implement changes in the organization it must implement plans that consist of activities that can make change in the organization to be effective. The plans can include creating or modifying structures and processes in the organization, loading, training and enforcement of new policies and procedures must be carried out so as to ensure that the changes can be implemented effectively.Organizational change efforts should be geared towards improving the performance of the organization and the people of the organization. There are tools know as system thinking and system theory this can enable an employee to explore, understand and communicate about the progress of change process effectively. An employee needs to unde rstand himself, his biases and know he manages feedback and conflict and how he makes decision so that he can be able to interact with the other people in the organization and can bring about good results to the organization. Change agents should be honest in their work and they should set good principles that will promote the successful implementation of change process.The organizational barriers that an organization faces are when the staff of organizations other neglects their obligations .In other circumstances change can’t be accomplished because the top officials fail to recognize and to manage the workers who contribute to the development of the organization the workers.Another factor that would lead to the process of implementing the changes of the business the employees are practices such as flexible job assignment in terms jobs rotations or flexible schedules this provides employees with a better overview of the organization and gives them the opportunity to improve and training would give employees a better understanding of the techniques used and potentially increase theirThe management may fail to explain the importance of change to the organization failure to provide information, failure of the employees to consult when they are encountered with problems failure of the employees to negotiate with other staff and failure of the employees to relate properly with other colleagues in the organization and failure of the top management to build trust and a sense of security to their officials   thus the employees may not have the know how of conducting their businesses. The top officials may ignore the ideas of the other junior staff since they fear that they will be intimidated by their juniors.In most cases change efforts fail because the managers fail to recognize that the way employees behave in different ways thus dealing with their behaviors can be a difficult task thus the management must be trained on the effective methods of implement ing changes in the organization,Due to changes that have occurred in the organization   the managers must properly structure their policies in the areas such in the rules and procedures of the organization,rewardsystems ,corporate identities and career planning programs .The management should at all times consult the experts when they implementing changes in the organization .The employees should change their attitude about how they conduct their activities so they can allow decisions of the organization to be implemented effectively.

Hamas Victory

The international community should continue with its restrictive policies towards Hamas even after it has recorded huge victories in the Palestinian’s election. Hamas has in the past declared its open hostility principle towards Israel its interests especially in the Middle East. Unless it openly denounces such ideas, it should continue to be treated as a terrorist group. Hamas is also referred to as the Islamic Resistance Movement. It practices both party politics and militarism. It is its later mission that has seen its branding as a terrorist organization by nations such as United States, Australia, Canada, United Kingdom and Israel amongst others. The European Union has also blacklisted it for exhibiting terrorist tendencies. It is currently having its moment of glory in Palestine having recorded surprise wins in the parliamentary elections that astounded many especially across the western world (Levitt, Matthew, 2006). The history of Hamas dates back to 1987. Its formation is known to have been inspired the Muslim brotherhood, a group reputed to have committed countless terrorist acts towards the Israelis. Hamas is said to have been formed by Sheikh Ahmed Yassin with a sole objective of annihilating the state of Israel and forcing its total withdrawal from the disputed Palestine territory. This is what its charter stipulates. It is credited for having been the mastermind and perpetrator of violence against the Israelis and their interests all over the world, these acts are meted out with impunity only equaled to that of al Qaeda. Together with Al Qaeda, Hamas leads the list of the worst terrorist organizations in the world (James L. Gelvin, 2005). After winning the elections in 2006 and assuming power, it issued a 10 year truce to Israel and vowing to let go temporarily, its suicide missions if the later withdrew from the disputed territories of Gaza, Jerusalem and West bank. This however was not the first truce as again in 2004, it had offered a truce if Israel could totally withdraw from the territories it captured during the Six Days War. This truce however was not held for long as it attacked Israel later in August 2005. Hamas had opted out of the presidential election in 2005 running for the municipal ones where it took control of some important territories. In the parliamentary elections the following year, it was able to garner over 42 percent securing majority seats. Hamas has been accusing Israel of violating the stipulations on the road map that had posited the formation of a Palestinian state, its feud is not limited to Israel only but also to the United States and is ready to wage terrorist attacks against the U.S in 2006 for example, a statement issued by the Hamas officials but later denied, urged the Muslims to target America and its interests all over the world, accusing it of providing financial and logistic support to the Israelis. Hamas officials though, have never owned up to any attack against the United States or to any other country apart from Israel (Lynfield, Ben, December 27, 2004). Its Democratic election into power has thrown a spanner in the works complicating relations with major powers in the world as well as the neighboring countries in the Middle East. Major countries in the western world have always branded Hamas as a terrorist organization. Japan for instance froze all Hamas properties within its jurisdiction in 2005. Jordan has in turn outlawed it due to its terrorist activities. All this animosity is directed to a political and terrorist organization that according to polls conducted in 2007 enjoys over 65% of popular support of Palestinians. The wide public support it has received stems from how it has initiated reforms in morale dilapidated country. It is rooting out massive corruption entrenched in the bureaucracy and order is being restored in the disputed territories. The bloody counter retaliations between the Hamas and Israel however continue, with both sides maintaining their hard stance. With grim prospects for a peaceful co existence, the issue of Hamas leadership in the Palestine state remains a headache for the western countries. Hamas has initiated a number of attacks this year killing a handful and maiming many in the region. It has perfected the art of guerilla warfare employing it tactfully against Israel. It is also accused of executions within its file and rank and also against its key rival, the Fattah. The United States however has been categorical in its stand even in the face of possible political realignments in the Palestine state. One state department spokesman said that the United States still maintains Hamas in its terrorist organizations’ list. The election has not changed this unless Hamas denounces its atrocious acts (Michael P. Arena, Bruce A. Arrigo, 2006). It is more than apparent that the United States is dismayed by the results but was quick to praise the temporal peaceful coexistence of people in Palestine. It is interesting to note that the Bush administration was non committal of the elections in Palestine as the prospects of Hamas loosing seemed grim. All this however is in the midst of reports in the media that the United States government together with president Mahmoud Abbas had planned to oust the Hamas leadership from the government. This was in an effort to overturn the gains made by Hamas. Hamas has been largely blaming the United States for funding and arming the Fattah combatants in its bid to water down its recent reigns (Tiebel, Amy, 2007). Some nations in Europe are caught between a rock and a hard place. There is a need to legitimize the democratic process that brought Hamas to power but also these countries are wary of an organization that has been labeled a terrorist group. Europe is divided. Berlin and the United Kingdom are adamant that they will have no relations with the Hamas. Russia however on the other hand welcomed Hamas leaders in Moscow, Turkey has also followed suit. Since its formation in 1987, Hamas has grown massively and seems to have stricken a chord with most Palestinians as evidenced in the latest elections. The greatest supporter of Hamas and financial bankroller is Iran. This is its vocal supporter but it has always denied claims of providing finances as alleged by the United States. The coming to power of the Hamas was met with elation from President Mahmoud Ahmadinejad of Iran who vowed to back the country financially. The United States claims that Hamas is funded by some Arab countries as well as private businessmen. Most of these countries maintain anti United States stand and are in support of any group that is likely to maintain hostility against Israel. The elevation of Hamas into power legitimizes the relations that Hamas may have had with those countries (Andersen, Mark, 2004). Iran has vowed to commit funds to help the democratically elected government in Palestine. Most of those countries that support Hamas are doing so on the claims of strengthening democracy and seeing it as a solution to the disputes in Middle East. It is obvious that the coming into power of Hamas has complicated the relations that most nations have had with it. Many are in a dilemma on whether to initiate diplomatic contacts with its members or to deal with the president directly. However putting into consideration what Hamas has always stood for, the stand by America and Germany is the way to go, no negotiations of any sort or diplomatic relations should be instituted until Hamas denounces its acts of terrorism and its stand against Israel. References Levitt, Matthew, 2006. Hamas: Politics, Charity, and Terrorism in the Service of Jihad, Yale University Press. James L. Gelvin, 2005. The Israel-Palestine Conflict: One Hundred Years of War, Cambridge University Press. Lynfield, Ben, December 27, 2004. Hamas gains grassroots edge, Christian Science Monitor. Andersen, Mark, 2004. All the Power: Revolution Without Illusion, Punk Planet Books. Tiebel, Amy, November 27, 2007. â€Å"Analysis: Tough Mideast Bargaining Ahead†, Associated Press. Mark Weitzman, Steven Leonard Jacobs,2003. Dismantling the Big Lie: the Protocols of the Elders of Zion, KTAV Publishing House. . Michael P. Arena, Bruce A. Arrigo,2006. The Terrorist Identity: Explaining the Terrorist Threat, NYU Press.

food of mexico essays

food of mexico essays Food is probably the most important element of Mexican culture. Much of the daily routine and tradition in Mexico revolves around the ritual of preparing and eating food. In history, women made their way to the local markets to fill their basket with vegetables, fruits, meat, and fish. Once collecting them the women would return home to begin grinding the corn and flour to make fresh tortillas for the afternoon meal. Mexican food is rich in color and flavor. The richness of their cuisine comes from their concern for the sensory experience of eating. It is often said that cuisine is culture, and to understand the development of Mexican cuisine it is important to know about the history of Mexico. In the pre-Colombian time corn was greatly used to make tortillas, tamales, or also made into flour. The diet of corn was placed with meats, vegetables, and many flavorful spices. However the countrys cuisine changed dramatically with influences brought by the Spanish. They brought rice, olives, wines, spices from India, and beef along with many others. The traditional food was combined with the spanish influence. This was the result of the blending of the two different heritages. In the 19th century, Mexican women played a important role in the home. To be a good woman in Mexico is to have extensive knowledge and great skills in the culinary arts. A testament to them, has been a demand for their wonderful dishes around the world. Mexicans are very proud of their cuisine. It gives them a sense of unity and identity everywhere in the world. ...

buy custom Trends in Recent American History essay

buy custom Trends in Recent American History essay Lyndon Johnsons efforts in promoting the great society programs were geared towards creating a positive environment for the poor and the minority in America. He wished to build a country free of disease, racial discrimination, poverty, uneven distribution of resources among others. This program also aimed at creating more job opportunities for the youths in America, who were at the verge of unemployment, at that time. For these programs to achieve their purpose, Johnson utilized his Presidential position to employ a number of strategies following the assassination of President Kennedy. First, he created a Declaration of War on Poverty by pushing for enactment of Economic Opportunity Act in early 1964. Actually, this Act availed funding for Job Corps, which guaranteed employments for the youths, residing in the inner city. It also established Head Start Program that provided early education opportunity for the disadvantaged preschoolers, as well as setting up a domestic version for the sake of Peace Corps, which were referred to as VISTA (Volunteers in Service to America). He also initiated Civil Rights Act to counter racial discrimination by bringing to end segregation in public facilities, creating Equal Employment Opportunity Commission charged with investigating channeled complaints of discrimination in job areas, and authorizing the attorney General to be able to file suits with the main aim of desegregating schools. How did the Vietnam War transform American politics and culture? The USs involvement in the Vietnam War was meant to contain any kind of spread of communism, especially in Southeast Asia. Therefore, the Unites States of America chose to give their full support to the anti-community regime, referred to as South Vietnam, in its bid to counter communist take-over. This involvement had a massive impact on American politics. First it became a stepping stone for Lyndon Johnson, to win election after serving 14 months, following the death of President Kennedy. His ability to continue working in Kennedys shoes (more so involvement in Vietnam) became a potential ground for his leadership quality to become inevitable; thus, the majority supported him at the time of election. Moreover, American involvement in Vietnam also raised a controversy over draft age, thus, lowering the minimum age required for an American voter to be 18, via the 26th Amendment. In addition, soldiers were also excluded from the political life, so that they could selflessly fight to th e extent of losing their lives, but did not have any say or influence on American politics. American culture was no exception, as it was infiltrated by the differing cultural perspectives. Certainly, the antiwar movement sparked a transformation in perception of the government by the American citizens, as well as questioning of their authority. These changes were also characterized by change in hair styles, genres of music, types of clothing, and types of films being acted in the country. What were the sources and significance of the rights revolution of the late 1960s? The Rights Revolution, experienced in late 1960s, in America, mainly originated from the African Americans. The American Civil Movement became the largest counter culture movement. This movement struggled to assure equal rights under the U.S. Constitution for all citizens of the United States of America. The African-American Civil Rights Movement championed outlawing of racial discrimination of the African Americans, and for the restoration of their voting rights. The enlarged goals of these movements were to enhance racial dignity, political and economic sufficiency and freedom from the yokes of oppression from the White Americans. These movements were of great significance in the history of United States of America, as well as to the welfare of the African Americans. The pressure surmounted by these movements led to abatement of racial segregation laws that have set a division of government services and public facilities into the separate colored and white domains. Surprisingly, the ones for the colored were in a poor state and were never funded. Disfranchisement was also outlawed opposite to the law that had disfranchised thousands of the African Americans, denying them of voting rolls, so that they could not even elect their representatives. The rate of exploitation was also put in its rightful place, as a result of these movements. Actually, these movements fought increased oppression along economic boundaries on Latinos, Asians and Blacks, as well as denial of economic opportunities and employment opportunities. In addition, the massive racial violence by organizations and police also reduced as a result o f pressure from these movements. What were the major policies of the Nixon administration on social and economic issues? The policies in Nixons administration were aimed at aiding parochial schools, supporting conducting prayers in schools, and opposing abortion, mainly, to appease the Roman Catholics, and partly, the Republican constituencies. Legal Segregation was declared to be inadmissible in this administration, leading to merging of the Black southern schools into unitary schools. In matters of economy, the administration provided employment openings and training for the minority groups in construction projects funded by the federal government. Besides, the Labor Department began using numerical goals and racial classifications, to implement desegregation programs, as an affirmative action. The Administration also proposed a program of family allowance and did away with nationalization of welfare programs, which instituted a minimum income through a system of negative tax income. What were the roots of the rise of conservatism in the 1970s? The roots of the rise of conservatism after 1970 are traced to have been a primary response to the 1960s excesses. As depicted in this chapter, they include changing roles of women characterized by a decline in nuclear families and rising of the US population to 205 million, mobilization of Evangelical Protestants characterized by a rise of mega churches, TV ministers and radio, evangelical ministers joining politics and pushing for conservative reforrms. Others include; economic changes of 1970s, characterized by Inflation Rising price of oil turn taxpayers against welfare programs, deindustrialization, and auto factories close, oil crisis of 1970s due to the Arab oil embargo, reaganomics characterized by Less Government regulations of business and opening wilderness to oil, gas, and timber industries, revival and end of the Cold War when Reagan turned his anti-communism toward El Salvador and Nicaragua, and then supplied weapons training to death squad. How did the Reagan presidency affect Americans both at home and abroad? Reagan presidency brought a great peace of mind to the Americans, whose country was engaged in the Cold War, whereby those abroad felt a huge sense of insecurity. Reagan through his symbiotic relationships managed to forge with Mikhail Gorbachev, the Soviet Union leader, at that time, leading to a peaceful resolution of the Cold War. Reagan declared this a world victory; therefore, enabling Americans feel a real sense of freedom and liberty. What were the major international initiatives of the Clinton administration in the aftermath of the Cold War? The main initiative taken by Clinton after the end of the Cold War, outlined in this chapter, is confronting problems that he considered to be complex, transnational, and diffuse making international environment, just as dangerous as the Cold War was. In respect to this, Clintons Administration resort to military intervention after defining their enemy in Somalia and Haiti, thus, resorting to constructing the primitive savage in Somalia, and defining modern savage in Haiti. What forces drove the economic resurgence of the 1990s? According to Eric Foner, the forces that drove economic resurgence in 1990s are the technological forces, whereby information technology relating to computer software, hardware and communication led to productivity growth. Political forces can also be credited to have enabled this resurgence. This is based on the fact that many people, in the government, and citizens, as well, became more enlightened at such a time, and they were able to understand the economy, thus initiating enactment of laws and legislations that enabled economic growth and productivity in the country. What cultural conflicts emerged in the 1990s? Foner depicts that a number of cultural conflicts emerged pertaining to varying moral coding among different groups in America. This included; reproductive rights/ abortion, politic identity, feminism, animal rights, legal drinking ages, homosexuality (gay rights and gay marriages), gun politics, family values, such as spanking (child discipline), and corporal punishment. Other forms of cultural conflicts related to prostitution, media bias, sexual revolution (abstinence only and sexual education), transhumanism, stem-cell research, secularization and secularism, moral relativism versus moral absolutism, multiculturalism, church separation, xenophobia and racism, recreational drug use, euthanasia and movement on right to die. Buy custom Trends in Recent American History essay

100 Major Works of Modern Creative Nonfiction

100 Major Works of Modern Creative Nonfiction Essays, memoirs, autobiographies, biographies, travel writing, history, cultural studies, nature writing- all of these fit under the broad heading of creative nonfiction, and all are represented in this list of 100 major works of creative nonfiction published by British and American writers over the past 90 years or so. Theyre arranged alphabetically by author last name. Recommended Creative Nonfiction Edward Abbey, Desert Solitaire: A Season in the Wilderness (1968)James Agee, Let Us Now Praise Famous Men (1941)Martin Amis, Experience (1995)Maya Angelou, I Know Why the Caged Bird Sings (1970)Russell Baker, Growing Up (1982)James Baldwin, Notes of a Native Son (1963)Julian Barnes, Nothing to Be Frightened Of (2008)Alan Bennett, Untold Stories (2005)Wendell Berry, Recollected Essays (1981)Bill Bryson, Notes From a Small Island (1995)Anthony Burgess, Little Wilson and Big God: Being the First Part of the Confessions of Anthony Burgess (1987)Joseph Campbell, The Hero With a Thousand Faces (1949)Truman Capote, In Cold Blood (1965)Rachel Carson, Silent Spring (1962)Pat Conroy, The Water Is Wide (1972)Harry Crews, A Childhood: The Biography of a Place (1978)Joan Didion, We Tell Ourselves Stories in Order to Live: Collected Nonfiction (2006)Joan Didion, The Year of Magical Thinking (2005)Annie Dillard, An American Childhood (1987)Annie Dillard, Pilgrim at Tinker Creek (1974) Barbara Ehrenreich, Nickel and Dimed: On (Not) Getting By in America (2001)Gretel Ehrlich, The Solace of Open Spaces (1986)Loren Eiseley, The Immense Journey: An Imaginative Naturalist Explores the Mysteries of Man and Nature (1957)Ralph Ellison, Shadow and Act (1964)Nora Ephron, Crazy Salad: Some Things About Women (1975)Joseph Epstein, Snobbery: The American Version (2002)Richard P. Feynman, The Feynman Lectures on Physics (1964)Shelby Foote, The Civil War: A Narrative (1974)Ian Frazier, Great Plains (1989)Paul Fussell, The Great War and Modern Memory (1975)Stephen Jay Gould, Ever Since Darwin: Reflections in Natural History (1977)Robert Graves, Good-Bye to All That (1929)Alex Haley, The Autobiography of Malcolm X (1965)Pete Hamill, A Drinking Life: A Memoir (1994)Ernest Hemingway, A Moveable Feast (1964)Michael Herr, Dispatches (1977)John Hersey, Hiroshima (1946)Laura Hillenbrand, Unbroken: A World War II Story of Survival, Resilience, and Redemption (2010) Edward Hoagland, The Edward Hoagland Reader (1979)Eric Hoffer, The True Believer: Thoughts on the Nature of Mass Movements (1951)Richard Hofstadter, Anti-Intellectualism in American Life (1963)Jeanne Wakatsuki Houston and James D. Houston, Farewell to Manzanar (1973)Langston Hughes, The Big Sea (1940)Zora Neale Hurston, Dust Tracks on a Road (1942)Aldous Huxley, Collected Essays (1958)Clive James, Reliable Essays: The Best of Clive James (2001)Alfred Kazin, A Walker in the City (1951)Tracy Kidder, House (1985)Maxine Hong Kingston, The Woman Warrior: Memoirs of a Childhood Among Ghosts (1989)Thomas Kuhn, The Structure of Scientific Revolutions (1962)William Least Heat-Moon, Blue Highways: A Journey Into America (1982)Bernard Levin, Enthusiasms (1983)Barry Lopez, Arctic Dreams: Imagination and Desire in a Northern Landscape (1986)David McCullough, Truman (1992)Dwight Macdonald, Against The American Grain: Essays on the Effects of Mass Culture (1962)John McPhee, Coming Into the Country (1977) Rosemary Mahoney, Whoredom in Kimmage: The Private Lives of Irish Women (1993)Norman Mailer, The Armies of the Night (1968)Peter Matthiessen, The Snow Leopard (1979)H.L. Mencken, A Mencken Chrestomathy: His Own Selection of His Choicest Writing (1949)Joseph Mitchell, Up in the Old Hotel and Other Stories (1992)Jessica Mitford, The American Way of Death (1963)N. Scott Momaday, Names (1977)Lewis Mumford, The City in History: Its Origins, Its Transformations, and Its Prospects (1961)Vladimir Nabokov, Speak, Memory: An Autobiography Revisited (1967)P.J. ORourke, Parliament of Whores (1991)Susan Orlean, My Kind of Place: Travel Stories from a Woman Whos Been Everywhere (2004)George Orwell, Down and Out in Paris and London (1933)George Orwell, Essays (2002)Cynthia Ozick, Metaphor and Memory (1989)Robert Pirsig, Zen and the Art of Motorcycle Maintenance (1975)Richard Rodriguez, Hunger of Memory (1982)Lillian Ross, Picture (1952)David Sedaris, Me Talk Pretty One Day (2000) Richard Selzer, Taking the World in for Repairs (1986)Zadie Smith, Changing My Mind: Occasional Essays (2009)Susan Sontag, Against Interpretation and Other Essays (1966)John Steinbeck, Travels with Charley (1962)Studs Terkel, Hard Times: An Oral History of the Great Depression (1970)Lewis Thomas, The Lives of a Cell (1974)E.P. Thompson, The Making of the English Working Class (1963; rev. 1968)Hunter S. Thompson, Fear and Loathing in Las Vegas: A Savage Journey to the Heart of the American Dream (1971)James Thurber, My Life and Hard Times (1933)Lionel Trilling, The Liberal Imagination: Essays on Literature and Society (1950)Barbara Tuchman, The Guns of August (1962)John Updike, Self-Consciousness (1989)Gore Vidal, United States: Essays 1952–1992 (1993)Sarah Vowell, The Wordy Shipmates (2008)Alice Walker, In Search of Our Mothers Gardens: Womanist Prose (1983)David Foster Wallace, A Supposedly Fun Thing Ill Never Do Again: Essays and Arguments (1997)James D. Watson, The Double H elix (1968) Eudora Welty, One Writers Beginnings (1984)E.B. White, Essays of E.B. White (1977)E.B. White, One Mans Meat (1944)Isabel Wilkerson, The Warmth of Other Suns: The Epic Story of Americas Great Migration (2010)Tom Wolfe, The Electric Kool-Aid Acid Test (1968)Tom Wolfe, The Right Stuff (1979)Tobias Wolff, This Boys Life: A Memoir (1989)Virginia Woolf, A Room of Ones Own (1929)Richard Wright, Black Boy (1945)

Molecular basis of RNA-dependent RNA polymerase II activity Coursework

Molecular basis of RNA-dependent RNA polymerase II activity - Coursework Example The RdRP activity has also been implicated in the replication of the RNA Genomes of HDV virus and plant viriods. 3) What is meant by HDV? Why would investigators want to study HDV? In your answer, tell me about HDV and why investigators would want to study HDV. What is the ANTIGENOME? Why is it important in the experimental design? HDV is Hepatitis Delta Virus. The investigators have taken HDV for the study as RdRP activity of Pol II has been established to be involved in the replication of the viral genome. Antigenome is the complementary positive RNA which serves as the template for the synthesis of the negative strand of the virus. To understand the physiological significance of the RdRP activity, the terminal segment of the HDV antigenome, which directs RNA synthesis and is sensitive to the Pol II inhibitor alpha- amanitin, was studied. 4) What is meant by â€Å"FAM-labeled RNA products were separated by denaturing gel electrophoresis†? DO NOT give me cookbook recipes, i.e. do not provide a ‘step-by-step how to’ that could be used to perform an experiment. Describe the method in general terms. FAM is 6 carboxy- flourescein label. Nucleotides labeled with such flourophores, have shown improved sensitivity, versatility and normalization when separated by denaturing gel electrophoresis. After purification of the nucleotides the fluorescein tag was attached by incubation with the 5’ terminal of the nucleotides. 5) DATA INTERPRETATION: Evaluate Figure 1. Break the analysis down. Strive to understand what each lane of the gel represents. Make note of the controls as well. Your focus for your response is the part of the figure labeled b, c, and d. Use part a in assisting you. Summarize what each part (b, c, and d) of the figure suggests. The RNA synthesized by the RdRP activity of Pol II is fluorescent labeled and separated by gel electrophoresis and viewed. Lane 1 in the gel shows only the reactant RNA.

Compare Rule and Act Utilitarians Paper Essay Example | Topics and Well Written Essays - 1500 words

Compare Rule and Act Utilitarians Paper - Essay Example It thinks more of the long term goals (Hayry, 2002). There are two forms of Utilitarianism. That is act Utilitarianism and other is rule Utilitarianism. They both are different and opposite from each other. Act Utilitarianism tells us that the basis of utility should be directly applied for each individual situation. In this, there is a point of view that the most happiness is for the greatest number of humans. The humans decide what to do after looking at that from where the most happiness is generated. In rule Utilitarianism it is told that what we should do to achieve the most wanted consequences and then the act that is seen is best for the majority is chosen (Lyons, 1965). In act Utilitarianism first the consequences are taken into sight of an act. The one with the better consequence is chosen. While the rule Utilitarianism looks at the consequence and then decide which rule to follow. Only that rule is selected that has the greatest utility or happiness whether the law is applied with fairness or not. Its aim is to deal people with fairness. It gives great value to justice. While in act Utilitarianism, it is to benefit the majority but sometimes the feelings are also hurt. Rule and Act Utilitarianism have one thing similar that they are interlinked with the prediction of future however; it is also a fact that predictions are not always right. This can lead to abrupt results which does not benefit the people. As act Utilitarianism is mainly concerned with gaining more good. The rights of the individuals are violated for benefiting the majority. But sometimes it happens that the decision taken is good but at the same time, it is thought that it is not beneficial for the people. It is because of the things that are changed (Crisp, 1997). Rule Utilitarianism constrains its usefulness. Rule Utilitarian believes that the rules should be followed as they benefit the majority (Hayry, 2002). While in

Persuasive paper Research Example | Topics and Well Written Essays - 1250 words

Persuasive - Research Paper Example By criticizing tobacco politics and highlighting financial burden, health hazards, and its prevalence, paper tries to persuade the readers about the danger of secondhand smoke and necessity of adopting effective laws and policies to protect people from secondhand smoke. On 18 June, 2002, Lynn French, a non-smoking flight attendant who worked for TWA airlines from 1977 to 1998 when smoking was still permitted in commercial flights, was awarded astonishing compensation of 5.5 million US dollars by the Miami Jury in a historic verdict against some of the leading cigarette manufacturing companies such as, Philip Morris, Reynolds Tobacco Holdings, Brown & Williamson Tobacco unit, and British American Tobacco (A WSJ News Roundup, 2002). The case against tobacco companies was filed under the accusation that Lynn French’s nonstop work in smoky, enclosed cabins of airlines caused significant negative impact on her health, including sinus problem. Lynn suffered from serious sinus infection due to the constant exposure to secondhand smoke, requiring long-term medical treatments (A WSJ News Roundup, 2002). The Lynn’s case is just another example of numerous victims of secondhand smoke all over the world, however, majority of them are not as lucky as Lynn in getting the justice. Today, the number of active smokers is rapidly increasing in the world. Consequently, at the same time, a large number of non-smoking populations, including children, has been exposed to secondhand smoke. Various researches and scientific studies have regularly highlighted serious negative effects of secondhand smoke on the physical and psychological health, yet the issue of secondhand smoking is widely neglected. The purpose of this paper is to emphasize on the severe negative effects of secondhand smoking and necessity of implementing effective measures against it. Before revealing health hazards of

A Concept of Citizenship Essay Example | Topics and Well Written Essays - 500 words

A Concept of Citizenship - Essay Example What is citizenship? A citizen is an integral participant of the social life. A political community consists of citizens, whose participation is directed on the state’s welfare. Citizens gain benefits from their nation and thus they have to follow laws and regulations of their country. There are many aspects in the concept of citizenship. On the one hand, citizenship is a benefit, which enables citizens to vote or to receive insurance etc. On the other hand, citizenship is a hard work, which introduces obligatory involuntarily actions, obtruded by the government on its citizens.  Ã‚  For example, citizens have to pay taxes, work in favor of the country, to get secondary education and many other obligations are invented by the state and must be followed by the citizens. Citizenship is a great responsibility. It is a hard work and every citizen should remember of his daily obligations, dictated to him by the state. There is no welfare of the country without daily obligations c ompleted by the citizens. Thus, it is evident that citizenship is an integral part of the country. It is a trigger of the state’s functioning. We can summarize that there is no way out from this scope of duties. Nevertheless, it should be noted that not all people living in the state are citizens.

Marketing Fundamentals Module (CIM Professional Certificate in Essay

Marketing Fundamentals Module (CIM Professional Certificate in Marketing) - Essay Example Their definition of a successful firm is one, which is able to predict the future accurately, acquire core competences ahead of competition and thereby become the dominant player within an industrial sector. Markets always change faster than Marketing. Today many companies are disappointed over marketing’s inability to produce measurable result. While companies unabashedly declare their wish to get closer to customers, marketing actually losing power to other functions in the corporation. Now to achieve highest level of operations every company needs a strong market plan, which will ensure success to them in the present competitive environment. 2. Marketing plan: The marketing plan operates at two levels strategic and tactical. The strategic marketing plan lays out the target markets and value proposition that will offer, based on an analysis of the best market opportunities. The tactical marketing plan specifies the marketing tactics, including product features, promotion merchandising, pricing, channels and services. The marketing plan is the central instrument for directing and coordinating the marketing effort. Today teams develop marketing plans with inputs and sign-offs from every important organizational function. These plans are then implemented at the appropriate levels of the organization, with management monitoring results and taking corrective action when necessary. The format and contents of marketing plans depend upon the size of the organization, the attitude of the organization the degree of formalization required within the annual planning cycle process and accepted sector conventions. 2.1. Situation review: Within situation review, there should be coverage of the strategic situation facing the organization. This will base on a description of market size, market growth trends, customer benefits

A Concept of Citizenship Essay Example | Topics and Well Written Essays - 500 words

A Concept of Citizenship - Essay Example What is citizenship? A citizen is an integral participant of the social life. A political community consists of citizens, whose participation is directed on the state’s welfare. Citizens gain benefits from their nation and thus they have to follow laws and regulations of their country. There are many aspects in the concept of citizenship. On the one hand, citizenship is a benefit, which enables citizens to vote or to receive insurance etc. On the other hand, citizenship is a hard work, which introduces obligatory involuntarily actions, obtruded by the government on its citizens.  Ã‚  For example, citizens have to pay taxes, work in favor of the country, to get secondary education and many other obligations are invented by the state and must be followed by the citizens. Citizenship is a great responsibility. It is a hard work and every citizen should remember of his daily obligations, dictated to him by the state. There is no welfare of the country without daily obligations c ompleted by the citizens. Thus, it is evident that citizenship is an integral part of the country. It is a trigger of the state’s functioning. We can summarize that there is no way out from this scope of duties. Nevertheless, it should be noted that not all people living in the state are citizens.

Prevention of mother to child Hiv transmission Essay

Prevention of mother to child Hiv transmission - Essay Example Quantitative research is the methodical scientific assessment of observable phenomena through computational, mathematical, or statistical methods. For example, the observable phenomena in health and social care may include HIV prevalence in expectant mothers, rate of mother to child HIV transmission, and infant deaths resulting from mother to child HIV transmission. Quantitative uses a systematic approach through actual data collection and analysis rather than making assumptions on describable phenomena. The purpose of the quantitative approach is to develop hypothesis about the observable phenomena. The research technique goes a long way in testing the hypotheses or theories so developed in order to ascertain their veracity or otherwise. In social and nursing care, scholars may use quantitative research to develop hypotheses around mother to child HIV transmission. The testing and proof of such hypothesis can bring significant benefit to the nursing fraternity (Daly, Speedy, & Jacks on 2009, pp. 135-140). Health and social care demands evidence-based practice, which quantitative research provides in hard data. In essence, evidence-based practice (EBP) or Evidence-based medicine (EBM) is the point at which the patient values & preferences, best research evidence, and clinical expertise converge. Evidence-based practice (EBP) involves the prudent utilization of the most reliable contemporary evidence in making critical decisions about the health of patients.

Civil War Essay Example for Free

Civil War Essay Brothers fighting brothers, a country imploding in itself, the world is an antithesis of what auspicious t it is today. A country separated by gender, race, religion, and class is that really a Union? The Civil War has begun, a fight not yet for the freedom of slaves but a fight to mold two sides of a coin into one. The oppressed brethren are reluctant to fight and why shouldn’t they be through all the injustices the Union has placed upon them? Regardless, Alfred M. Green delivers a speech in an effort to persuade his brethren into fighting for the right to join the army that empathizes with his fellow brethren’s inundation, that promises commemoration and recognition for his fellow African Americans, and that promotes the Union’s battle as a righteous one. Green starts his speech down on the mat with a crowd that holds the candid opinion of not wanting to join a fight for a country that oppresses its people, enslaves its people, and imprisons its people. Nevertheless Green acknowledges his brethren’s suffering and injustice caused by the â€Å"fugitive slave-laws† and the â€Å"Dred Scott decisions† and states that ‘[their] duty is not to cavil over past grievances† but to aid the country with â€Å"burning zeal and enthusiasm†. By empathizing with the injustices the Union has committed towards the black community, Green preemptively dismisses the crowd’s probable argument and establishes a common ground to build upon later in his speech. Likewise, Green mention’s that â€Å"the brave deeds of our fathers†¦ have failed to bring us recognition† which quickly refutes the future argument that the Brethren have already sacrificed their share and establishes the point that it’s up to the blacks of that era to bring â€Å"honor† and â€Å"glory† to the African American race. After acknowledging the mistreatment the Union has committed to the free and enslaved blacks, Green emphasizes that joining the war against the South will â€Å"improve the present auspicious moment creating anew [the brethren’s] claims upon the justice and honor for the Republic†. This quote suggests that taking up arms for the Union will secure the future of free African Americans with freedom and honor. In addition, Green says, â€Å"let not the honor and glory achieved by our fathers be blasted or sullied by a want of true heroism among their sons†. Green invokes â€Å"filial piety† in the crowd by ultimately saying that the good reputation their ancestors had laid out in the Revolutionary War and the War of 1812 should not be â€Å"sullied† or â€Å"blasted† and should be preserved and built upon for this time they may secure a free future. Towards the climax of his speech, Green promotes the Union Army as God’s Army and â€Å"[he or God] will defend the right, remembering that these are other days than those of yore.† By stating God wants the audience to â€Å"take up the sword† ultimately encourages the religious brethren to join this â€Å"army of God† and gives these mistreated people hope because God wants them to succeed and gain freedom from judgment and racism imposed on their race. Green also says â€Å"[their] very presence among the troops of the North would inspire [their] oppressed brethren of the South with zeal†¦ and confidence.† Green’s intention for this quote was to evoke the righteousness of the northern audience to fight for the southern slaves who are enduring far worse hardships. Alfred M. Green recognizes the hardship his race has endured but insists that their involvement in the war will secure a future filled with honor and glory and insists that God sides with the North and trusts them to fight for and free the enslaved south. In essence, Green convincing blacks and whites to work together as equals was the leap of mankind that inspired the many immigrants and future generations to see the United States of America as the land of freedom and the home of the brave.

Strains of ESBL Producing E. Coli | Investigation

Strains of ESBL Producing E. Coli | Investigation Introduction Background of Study Extended Spectrum Beta- Lactamases (ESBL) are beta lactamases which are mainly produced by family members of Enterobacteriaceae derived from mutations of the previous broad-spectrum beta-lactamase (Sharma et al., 2010). This enzyme works by hydrolysing and destroying the ÃŽ ²- Lactam ring of all cephalosporins, penicillins and monobactams (Sharma et al., 2010). In recent years, the emergence of ESBL producing Escherichia coli has posed a very serious problem to the management of diseases caused by this organism as only limited choice antibiotics can be given to patients. Carbapenems are the drugs of choice for the treatment of ESBL producing E.coli, however, carbapenamase resistance has recently been reported (Paterson and Bonomo, 2005). Prolonged use of antibiotics was suggested as the main cause of the emergence of ESBL E.coli and the fact that the genes coding for ESBLs are easily transferred from one organism to another organism via conjugation, transduction and transformation ma ke the spread even quicker (Vaidya et al., 2011). ESBL producing organisms were first reported from a patient in Germany in 1983 and since then , several outbreaks have been reported worldwide usually one particular â€Å"super† strain has been involved presumably combining not only the capability to produce ESBLs but also possessing various other virulence factors that contribute to their pathogenic success. (Harada et al., 2013). These pathogenic ESBL producing Escherichia coli in recent years have become a major concern and their emergence is now become alarming in clinical fields and subjected to comprehensive studies worldwide. The most common infections caused by pathogenic ESBL producing E.coli are urinary tract infections (UTI), bloodstream infections, gastrointestinal infections (Fatima et al., 2012; Bekat et al., 2002). According to Petty et al., (2013), globally, E.coli sequence type ST131 is the multidrug resistant clone strain which is responsible for ESBL CTX-M15 bearing genes, and it is the most alarming pathogenic ESBL producing E.coli associated in causing UTIs and septicaemia in hospital community acquired infections. ? in UK or worldwide? As genes coding for ESBL in Escherichia coli are known to be transferable this raises further fear of the spread of these genes to other strains, continuous monitoring of the predominant strains of E.coli which carry the ESBL genes is therefore important. Problem statement Studies of ESBL producing Escherichia coli in the South Manchester population have been carried out previously. This study will investigate strains of ESBL producing E. coli currently circulating in the Stockport Population of South Manchester and compare them to those delineated in the previous studies using a molecular typing and pulse-field gel electrophoresis. Objectives The objectives of the project are: Screen for ESBL Escherichia coli clinical isolates Identify strain using PFGE Assess the relatedness of the strains by PFGE analysis Determine Escherichia coli plasmid profile Identify Escherichia coli phylotyping group 1.0.4. Significance of study Finding from this study will contribute to the existing data and the body of knowledge on the molecular relationship of predominating of E.coli isolates from South Manchester populations. 1.0.5. Scope and Limitations There are no data on the antibiotics consumed by the patients in which the clinical isolates originates from. The availability of this data might help in understanding relationship between an exposures of certain antibiotics to the emergence of ESBL producing E.coli strain. PFGE also has several limitations in which the method assess visual relatedness of an isolates and not using a phylogeny relationship which provide more accurate molecular relationship between an isolates. Escherichia coli Escherichia coli is a motile gram negative rod, facultative anaerobe, non- spore forming bacteria taxonomically belong to the family of Enterobacteriaceae. It is considered as a normal inhabitants of gut and intestine in almost all warm blooded mammals and found as a faecal contaminant in the environment (Brennan et al., 2010; Darnton et al., 2007; Diniz et al., 2005). Most varieties of E.coli are harmless and do in the most part contribute to the normal and healthy intestine condition, while a few cause limiting abdominal cramp associated with diarrhoea. However, there are some serotypes that becoming a major threat to the human health, because they have acquired certain genetic material and virulence factors which enabling them transformed into pathogenic E.coli causing broad spectrum of disease (Clarke et al., 2003; Kaper et al., 2004). Pathotypes of E.coli are classified by specific mechanism in which they causing a disease, presence of certain virulence genes and their clinical manifestations (Chang et al., 2004). Growth requirements E.coli are non- fastidious bacteria, thus it can be cultured in artificial media with various altered physical and nutritional growth factors. It can be isolated easily from clinical samples by culturing into culture media and incubated at optimum temperature of 37 ºC anaerobically or aerobically as it is a facultative organisms (Yunlin et al., 2004) Uropathogenic Escherichia coli According to Pitout et al., (2005) E. coli is a frequent cause of the urinary tract infections (UTIs) of a hospitalised and non- hospitalised patients. UTIs are usually self- limiting but untreated lower urinary tract infections such as simple cystitis (bladder infection) can lead to much more severe illness known as pyelonephritis (renal infections) mainly among adult women (James et al., 2011). Infections occur by ascending movement of E. coli up the periurethral area colonising the bladder or infections by movement down from the intestinal tract. Due to anatomical complexities in women, they are more prone to be diagnosed with UTIs for at least once in their lifetime (James et al., 2001) 1.3  Escherichia coli typing 1.3.1  Plasmid profiling Multidrug resistant bacteria including ESBL producing Escherichia coli acquire their resistance by various gene transfer mechanisms which include transformation, horizontal transfer either by transduction, and conjugation, transposon and most often, are plasmid mediated (Carattoli et al., 2005) Plasmids are an extra chromosomal fragments of self- replicating DNA present in most of the bacterial species. Plasmids contain genes that are an essential for the replication of genes that promotes resistance to agents such as antibiotics, ultraviolet radiation, metals and bacteriophages. 1.3.2  Pulse-field gel electrophoresis PFGE was developed and described first by Schwartz and Cantor (1984). It is a molecular technique of typing a bacteria especially pathogenic Escherichia coli 0157:H7, non 0157: H7, Salmonella serotypes, Shigella sonnei and Shigella flexneri. PFGE uses a gel electrophoresis- based technique that allows separation of large molecular weight DNA up to 2Mb- 10Mb using a standard PFGE method (CDC, 2013; Hansen et al., 2002; Vimonet et al., 2008) PFGE is different to conventional gel electrophoresis as the large genomic DNA is digested with restriction enzyme that recognise and cleave specific sequences of DNA known as restriction site in an organism to produce a multiple DNA fragments which differ in size of their molecular weight (Van der Ploeg et al., 1984). The fragments are then run through constant changing electric field of PFGE resulting in a formation of DNA at various discrete size bands. This typing method has also been shown to have more discriminating power and reproducibility between laboratories than the newer molecular typing method such as ribotyping and multi- locus sequence typing (MLST) which confer more on the global epidemiology and revolutionary relationship between bacterial species (Vimonet et al., 2008) 1.3.3.  Escherichia coli phylogenetic group 2.0  Materials and Methods 2.0.1  Bacterial Isolates Bacterial isolates used in this study were Escherichia coli clinical isolates which was collected from Stepping Hill Hospital. Isolates undergo an anonymisation numbering of 1 to 20. 2.0.2.  Bacteriological Media The media used in the study were a selective differential medium for UTI Escherichia coli which is Chromogenic agar and nutrient agar which was used as a medium for growth and maintenance of isolates. 2.0.3  Antibiotic disks Table 1: Antibiotic disks used in this study was obtained from Oxoid.Ltd. Antibiotics Antibiotic Group Gentamicin (10 µg) Aminoglycosides Ciprofloxacin (5 µg) Quinolone Amoxicillin (25 µg) Penicillin Cefpodozime (10 µg) Cephalosporin Mecillinam (10 µg) Beta lactam Trimetophrim (2.5 µg) Bacteriostatic ESBL Disk kit (Mast Diagnostics) 2.0.4  Buffers and solutions Tris Borate EDTA (TBE X1 and X0.5) (Sigma) pH 8.2 was used as a running buffer in agarose gel electrophoresis. 2.0.5  Commercial kits The commercial kit used in this study was QIAprep Spin Miniprep Kit (Qiagen) and DNeasy Blood and Tissue Kit (Qiagen) 2.1.  Screening for multidrug resistance and potential ESBL producers in Escherichia coli clinical isolates Antibiotic susceptibility of Escherichia coli to six antibiotics (Table 1) were tested using the Kirby Bauer disk diffusion method. A 24 hour cultures from Nutrient agar was used. Then, a single colony was taken and transferred into 5ml Mueller Hinton Broth. It was then incubated at 37 °C to develop a heavy suspension of overnight cultures. A sterile cotton swabs were used to streak onto the Mueller Hinton agar and the rotation were repeated for three times. A final sweep was made around the rim of the agar. The plates were allowed to dry for several minutes. Using antibiotic dispenser, the disk that has been impregnated with a fixed antibiotic concentration was placed on the surface of the agar surface. After 24hr of an incubation period, the plates were checked for the presence of inhibition zone. Each recorded inhibition zone was compared with antimicrobial susceptibility testing disc chart provided by The British Society for Antimicrobial Chemotherapy (BSAC). The inhibition zon e of each antibiotic was reported as ‘sensitive’, ‘intermediate’ or ‘resistance’. Isolates showing resistance to three or more classes of antibiotics were considered as multidrug resistance (Falagas, 2007). ESBL producers were detected by testing sensitivity of isolates against a pair discs (cefpodoxime 10 µg and cefepime 10 µg) with and without clavulanic acid placed oppositely on an agar. According to manufacturer (Mast diagnostics), isolates were considered as an ESBL if there is a presence of 5mm larger inhibition zone in disks with clavulanic acid rather than the disks without the clavulanic acid. 2.2. Determination of plasmid profiles in MDR and ESBL Escherichia coli 2.2.1  Plasmid Extraction Prior to Plasmid DNA extraction, a fresh overnight cultures of E.coli after an incubation at 37 ºC in a Mueller Hinton broth were harvested. Plasmid DNA extraction was carried out using QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s instructions. Extracted plasmid DNA was stored at -20 ºC until use. 2.2.2  Detection of plasmid by agarose gel electrophoresis The profiles of the plasmid DNA was determined on a 0.7% agarose gel electrophoresis which has been carried out at 70 Vcm-1 for 120 minutes. The size of DNA bands was estimated using Hyper ladder 1 (Bioline) as a reference molecular weights marker. The bands were visualized under UV transilluminator and photographed with digital camera connected to visualisation unit (Alpha Innotech) and the size of the plasmid were measured by visual comparison to the reference marker. 2.3  Escherichia coli pathotypes determination 2.3.1.  Genomic DNA extraction Primary cultures on the nutrient agar was inoculated into 3ml Mueller Hinton broth for 24 hours at 37 ºC. The cells was then harvested by centrifugation at 12, 000 for 3 minutes. Genomic DNA extraction was carried out using DNeasy Blood and Tissue (Qiagen) kit following the manufacturer’s instructions. Final volume of 150 µl genomic DNA were collected and kept at -20 ºC until needed. 2.3.2  Multiplex PCR for Escherichia coli phylotyping PCR reaction mix preparation must be carried out on ice. PCR was performed in 0.2ml PCR tubes on a GeneAmp PCR System 9700 thermocycler (Applied Biosystems ®) with a total 25 µl of reaction volume as described in Table 2 and PCR condition according to Table 3. The negative control reaction lacking the DNA was included. Table 2:  PCR reaction mix Components Required concentrations Volume ( µl) per reaction Biomix Red 2X 12.5 Primer (forward) chuA yjaA tspE4.c2 20pmol 20pmol 20pmol 1 1 1 Primer (reverse) chuA yjaA tspE4.c2 20pmol 20pmol 20pmol 1 1 1 DNA 2 Ultrapure sterile water 4.5 Total volume per reaction 25 Table 3: Conditions for PCR gene amplification Genes Primer sequence PCR condition chuA Forward 5’-GACGAACCAACGGTCAGGAT-3’ Reverse 5’-TGCCGCCAGTACCAAAGACA-3’ Initial denaturation: 94 °C for 4 mins Denaturation: 94 °C for 25 secs Annealing: 52 °C for 40 secs 30 cycles Extension: 72 °C for 50sec Final extension: 72 °C for 6 mins yjaA Forward 5’-TGAAGTGTCAGGAGACGCTG-3’ Reverse 5’-ATGGAGAATCGGTTCCTCAAC-3’ tspE4.c2 Forward 5’-GAGTAATGTCGGGGCATTCA-3’ Reverse 5’-CGCGCCAACAAAGTATTACG-3’ 2.3.3  Detection of by agarose gel electrophoresis After completion of the multiplex PCR, the amplification product were separated by dry electrophoresis system. 15 µl of amplified product was mixed with 5 µ 5X DNA loading buffer (Bioline) and loaded onto 2% agarose gel incorporated with SYBR green dye. After electrophoresis, the gel was visualised by exposing the gel under UV light and was photographed with a digital UV camera connected together with the visualisation unit (AlphaInnotech). The size of the amplicon were measured by visual comparison to the 1kb DNA marker (Bioline). Phylogenetic typing analysis were carried on the basis of the presence or absence of an amplicon sized 279bp, 211bp and 152bp which belong to chuaA, yjaA and tspE4.c2 genes respectively. 2.4.  Pulse- field gel electrophoresis (PFGE) 2.4.1.  DNA extraction Each isolates was inoculated into 5ml Mueller Hinton Broth and incubated overnight at 37 ºC with gentle agitation. Cells were then harvested by placing 1ml of culture into 1.5ml microcentrifuge tube and was centrifuged at 13, 000 rpm for one minutes. The supernatant was discarded and the process was repeated until all the 5ml of culture finished. The supernatant was again discarded and pellet of cells was resuspended in 500 µl of 0.5M EDTA buffer (see appendix) and was centrifuged at 13, 000rpm for one minutes to removes broth debris that might be interfering with the extraction processes. The washing step was repeated twice to ensure complete removal of debris. The supernatant was discarded once again and pellet was resuspended in 500 µl of suspension buffer. 2.4.2.  Preparation of low melting point (LMP) agarose To prepare the LMP agarose, 3g of SeaKem PFGE agarose (BioRad) were dispensed into 100ml of TE buffer (see appendix) in a universal bottle. It were then heated to dissolve. Agarose was transferred to a 56 ºC waterbath until needed. 2.4.3.  Preparation of the bacterial plugs The wells of PFGE plug molds were numbered. 3 plugs was prepared for each isolates. Then, 750 µl of LMP agarose was added immediately into each cell- buffer suspension and carefully mixed by pipetting up and down several times and be careful not to induce any formation of bubbles. The mixture of cells and agarose was quickly pipetted into the well of a plastic PFGE plug molds (BioRad). The wells was filled to the rim and plugs were allowed to solidify at room temperature or chilled for 5 minutes in the refrigerator. 2.4.4.  Lysis of the cells The cells were lysed by adding a mixture of 1ml of proteolysis buffer with 10 µl of Proteinase K stock solution (50mg/ml) (see appendix) into a 1.5ml new labelled microcentrifuge tube. The plugs were removed from the plug molds by peeling the sealant tape below the wells until all tape was removed. The PFGE plastic arm was used to push the plugs out of the molds into the microcentrifuge containing the mix of proteolysis buffer-proteinase K solution. All plugs for one isolates were transferred into the same tubes. Care was taken while pushing the plugs out of the molds as not to tear the fragile plugs. Tubes was then incubated in a heating block at 50 ºC for 24 hours for digestion to take place. 2.4.5.  Washing of the plugs After completion of an overnight incubation, the proteolysis buffer and Proteinase K activity were eliminated by carefully pipetting out the volume, care taken not to tear the plugs. The plugs were then washed with TE buffer. The washing steps was repeated three times, for every half an hour and were held at room temperature to equilibrate the plugs. 2.4.6.  Restriction enzyme digestion After completion of the washing steps, wash buffer was removed in the final wash leaving only agarose gel in the tubes. Then, 300 µl of 1X restriction enzyme buffer specific to the enzyme used was pipetted in each tubes containing the agarose plugs and was let to equilibrate at room temperature for 10 minutes. The restriction buffer was then discarded, taking care not to tear the plugs. Next, 300 µl of restriction buffer containing 50U of Xbal enzyme was added into the tubes and was incubated in an incubator for 24 hours at 37 ºC specific to the optimal temperature for Xbal enzyme. 2.4.7.  Pulse- field gel electrophoresis 2.4.7.1.  Electrophoresis gel preparation. After incubation, restriction enzyme reaction was stopped by addition of 200 µl of 50mM EDTA. Plugs were cooled at 4 ºC until needed. Then, a (1%) agarose gel was prepared by heated to dissolved 3g of PFGE grade agarose (BioRad) into 300ml of 0.5X TBE buffer over magnetic hot plate with constant stirring or in the microwave and swirl to dissolved. The agarose was then poured into a casting tray that has been placed with PFGE comb and let to solidify at room temperature. The enzyme- buffer was aspirated and one plug of each isolates was loaded into the gel. Care was taken not to tear the plugs. Then, a thin slice high range and mid- range lambda molecular weight marker (New England Biolabs) was loaded into the wells next to each other. After all samples was loaded into wells, the wells were sealed with melted LMP agarose. 2.4.7.2.  Electrophoresis Run The electrophoresis was performed by using a CHEF mapper (BioRad) which subsequently was filled with approximately 3 liters of 0.5ml TBE buffer. The running buffer was let to cool approximately at 14 ºC before turning on the pump. The run time was set for 24 hours at 6 Vcm-1 with 120 º angle using switch time of 2.16 sec to 54.17 sec. 2.4.7.3.  Gel staining Once the run was complete, the gel was stained with 3X Gel red nucleic acid stain (Biotium) with approximately 200ml distilled water and was gently agitated on rotary shaker for 20 minutes. The gel was then visualised under UV transilluminator and a picture was taken once optimal image obtained.

Signs Essay -- essays research papers

  Ã‚  Ã‚  Ã‚  Ã‚  The movie â€Å"Signs† by M. Night Shyamalan is an excellent movie that explains how reflections and occurrences of the past can influence your overall perceptions and views of the future. It takes place in a small farm town where everyone knows each other very well. A single father is forced to continue raising his two young children due to the tragic death of his wife who was involved in a fatal car accident. This causes him to resign from his preaching career and become a stay at home farmer due possibly to his question of faith. Being a preacher, this man must have thought that nothing would have ever happened to him because of the way that he reacted to the stimuli. He was probably one preacher of only a few in the whole area. With the arrival of extra terrestrials all over the globe, including in the corn crops of his farm, this already upset father ends up with his hands full of problems that he is unwilling to accept and face. It is hard to understa nd what this man has had to deal with. There is an extreme lack of communication in this household and something happened for a reason to bring them back together and much more tightly knit. The presence of the uncle has been positive and well received during these tough times for the whole family. There are many theories that can describe what is happening. They are the attribution theory, causal attribution theory and the social penetration theory which will be included in the following paragraphs.  Ã‚  Ã‚  Ã‚  Ã‚     Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  First of all, the social penetration theory is a theory that we as people disclose to others in stages like pieces of a pie. Each piece is different facets of self and has the potential to be superficial or deep. It was very interesting to analyze how things were, or more the less weren’t communicated in this seemingly lifeless household. The children did not talk or play like most children grow up doing. There was a blank stare on their faces most of the time and their voices were monotone. The father would experience flashbacks of the incident where his wife was killed which was sending him a message that he needed to change his ways of non communication towards his children. Even in the case of traumatic events, it is necessary to talk about them to ensure closure and strength to the survivo... ...the children live their lives the way that they should. He was punishing himself as well as his surroundings for something he could not have controlled. A main point that his wife made for him which he recalled was that everything happens for a reason. That is what triggers his change of attitude and communication skills.   Ã‚  Ã‚  Ã‚  Ã‚  Overall, this movie made me realize how important interpersonal communication is. If there were to be a sequel to this movie, I think that there would be a significant change in communication between everyone. Especially after the father is faced with the possibility of his son dying because of an asthma attack. There are many theories that can explain what causes the actions and behaviors in the movie. The one that best explains what is going on is the attribution theory because of the given circumstances and how the events that occurred caused a chain reaction that affected everyone in the family. It created a snowball effect of denial until finally the father decides that he must stand up and face reality. Interpersonal communication is and will always remainideal to the success in families whether they suffer from trauma or not.

Michael Oher Essay

African-American Michael Oher’s story started when he ran away from home, escaping his drug-crazy mother who was fighting her own battle of maintaining a husband-less family and retaining money for drugs, food, and other â€Å"necessities. † Although he kept poor grades up until the year he graduated from high school, Oher showed an aptitude in sports from an early age. This would play a major role in his future, as we now see him playing as a tackle for the Baltimore Ravens. In his freshman year of high school, Oher was living temporarily with an auto mechanic whose son was just about the same age as him. His guardian enrolled both his son and Oher into Briarcrest Christian High School so they could study together. However, he ran into financial troubles and had to force Michael to leave. Eventually, Oher ran into Sean and Leigh Anne Tuohy, who adopted him and took care of him until he went to college. Briarcrest Christian was a predominantly white school; in fact, the entire district was a predominantly white district. This meant that because Oher was African-American, he faced a lot of racial tensions in his school. He had to deal with countless people staring at him or talking about him when he was walking down the hallways in school. In fact, his mother’s friends and his sister’s friends were discouraging them both to keep him as a foster son. In football games, Oher faced many racist people on his team and from opposing teams. However, he was able to brave through many of the slurs thrown at him and channel a lot of his anger into football. By the end of senior year, Oher received varsity letters in football, basketball, and track-and-field. He received scholarships from many colleges including Tennessee, LSU, Auburn, Alabama, and South Carolina, but Oher finally decided on going to the University of Mississippi, where his foster parents and his tutor went to. He primarily went so he could play on the Ole Miss Rebels football team. We can parallel many of Oher’s actions to his baseball counterpart—Jackie Robinson. Jackie Robinson endured many of the taunts and racial jeers that were hurled at Oher throughout his high school life. Similarly, both of these people rose up against their problems to surpass the white people that made fun of them before—Robinson being nationally ranked as one of the best baseball players of his time, Oher being one of the best left tackles in his team in high school.

Gender Role and Sexuality Essay

I can fortunately and happily say that I personally have not experienced unequal treatment in regards to my chosen career, educational path or previously held jobs. What I do still see currently being stereotyped are gender roles and the way they are allowed or not allowed to sexually express themselves in the public eye. From my experience it is more acceptable for men to compose music about sex, be in main stream media selling sex, have successful careers in sex, and everyday unequal expectations in dating, sex and relationships. Men have more sexual freedom in the world today. Men are encouraged and in most cases applauded on the discussion of how many partners they have had in the past and the specifics of each experience, yet woman are still expected to hide their sex from the world in fear of ridicule and isolation. In our society today woman who are overly sexual or dress in form fitting clothes or lack thereof are automatically stereotyped as the promiscuous type. Why the double standard? I don’t agree at all with displaying private sexual acts to the general public to gain fame and wealth and in by doing such corrupting children and destroying the beauty of sex but I don’t believe that we should have to hide that women are as much sexual beings equally as men. I feel religion and culture play major roles in the history of sexual repression of woman. Even dating back to the Puritan who believed that anything resulting in pleasure was a sin, women’s freedom to express their sexuality has been suppressed in fear of domination because we are just as powerful as men. Growing up in a strict catholic home I was always taught to act like a lady in public. Exactly what was meant by that I wasn’t fully sure but I knew its underlying symbolism was repressing my sexuality. I wasn’t allowed to wear spaghetti straps, mid drift shirts, shorts above my knees, or make up till I was 18 years old. Sex was not spoken of in my household at all, and if it happened to be seen in a movie or on television I was instructed to cover my eyes because sex was forbidden. Being a sexy woman was seen as vulgar. If I had male friends I could not talk on the phone with them or play outside of school with them without a parent or chaperone. On that contrary my younger brother was encouraged to dress well, wear cologne and have many female friends. It was a symbol of popularity. In my father’s Hispanic culture the young men were pampered and treated as kings with double standards. The women were there to serve them. We are not allowed to be sexy or let alone discuss sex in anyway shape or form. As an adult now I can understand why I completely rebelled against the stereotypes created by my religion and culture. It’s not fair to make women feel less than human than men. We are sexual creatures who share the same desires and needs. I now embrace my sexuality. I’m not afraid to be who I am, and say how I feel. Being a single mother in society today puts me in another stereotype as well. Having a child out of wedlock is still looked down upon, and more so with the mother. We are labeled as â€Å"promiscuous†, â€Å"unholy†, and â€Å"irresponsible†, but to me I loved a man for 8 years and wanted to create a life with him. Unfortunately the abuse I endured was too great to allow after the child was born, so I chose to leave. On the other hand the single father is praised and labeled as â€Å"hard working†, and â€Å"honorable†. In news and media woman are portrayed as sex objects in order to entice society. It draws women in because they either want to be like them or look like them and it draws men in because they love the visual stimulation. Sexuality is used as a shock value for ratings. Are sexy woman who love their bodies and the way it makes them feel really that shocking? Instead of conditioning our young woman to hide their sexuality we should be teaching them healthy ways to explore and express their human desires of sexuality in a safe, healthy way.

Bombay Stock Exchange

Bombay Stock Exchange – Wikipedia, th†¦ Coordinates: 18. 929681 °N 72. 833589 °E Bombay Stock Exchange From Wikipedia, the free encyclopedia The Bombay Stock Exchange (BSE) (Hindi or Marathi: Bombay Share Bazaar) (formerly, The Stock Exchange, Bombay) is the oldest stock exchange in Asia and largest number of listed companies in the world, with 4990 listed as of August 2010. [2][3] It is located at Dalal Street, Mumbai, India. On Aug, 2010, the equity market capitalization of the companies listed on the BSE was US$1. 39 trillion, making it the 4th largest stock exchange in Asia and the 11th largest in the world. 4] With over 4,990 Indian companies listed & over 7700 scrips on the stock exchange,[5] it has a significant trading volume.The BSE SENSEX (SENSitive indEX), also called the â€Å"BSE 30†, is a widely used market index in India and Asia. Though many other exchanges exist, BSE and the National Stock Exchange of India account for most of the trading i n shares in India. Bombay Stock Exchange Contents 1 Hours of operation 2 History 3 Timeline 4 BSE indices 5 Sensex correlation with emerging market indices 6 Awards 7 See also 8 References 9 External links Type Stock Exchange Location Mumbai, India Coordinates 18. 29681 °N 72. 833589 °E Founded 1875 Owner Bombay Stock Exchange Limited Key people Madhu Kannan (CEO & M D) Currency Indian rupee No. of listings 4,996 MarketCap US$1. 39 trillion (August 2010)[1] Volume US$980 billion (2006) Indexes BSE Sensex Website www. bseindia. com (http://www. bseindia. com/) Hours of operation Session Trading Session Timing 9:00 – 15:30 Beginning of the Day Session 8:00 – 9:00 †¦wikipedia. org/†¦/Bombay_Stock_Exch†¦ 1/6 9/27/2010Bombay Stock Exchange – Wikipedia, th†¦ Position Transfer Session Closing Session Option Exercise Session Margin Session Query Session End of Day Session 5:30 – 15:50 15:50 – 16:05 16:05 – 16:35 16:35  œ 16:50 16:50 – 17:35 17:30 The hours of operation for the BSE quoted above are stated in terms of the local time (i. e. GMT +5:30) in Mumbai (Bombay), India. BSE's normal trading sessions are on all days of the week except Saturdays, Sundays and holidays declared by the Exchange in advance. [6] History The Bombay Stock Exchange is the oldest exchange in Asia. It traces its history to the 1850s, when 4 Gujarati and 1 Parsi stockbroker would gather under banyan trees in front of Mumbai's Town Hall.The location of these meetings changed many times, as the number of brokers constantly increased. The group eventually moved to Dalal Street in 1874 and in 1875 became an official organization known as ‘The Native Share ; Stock Brokers Association'. In 1956, the BSE became the first stock exchange to be recognized by the Indian Government under the Securities Contracts Regulation Act. The Bombay Stock Exchange developed the BSE Sensex in 1986, giving the BSE a means to measure overall performance of the exchange. In 2000 the BSE used this index to open its derivatives market, trading Sensex futures contracts.The development of Sensex options along with equity derivatives followed in 2001 and 2002, expanding the BSE's trading platform. Historically an open outcry floor trading The Phiroze Jeejeebhoy exchange, the Bombay Stock Exchange switched to an electronic trading system Towers house the Bombay in 1995. It took the exchange only fifty days to make this transition. This Stock Exchange since 1980. automated, screen-based trading platform called BSE On-line trading (BOLT) currently has a capacity of 80 lakh orders per day. The BSE has also introduced the world's first centralized exchange-based internet trading system, BSEWEBx. o. in to enable investors anywhere in the world to trade on the BSE platform. [7]. The BSE is currently housed in Phiroze Jeejeebhoy Towers at Dalal Street, Fort area.Timeline Following is the timeline on the rise and rise of the S ensex through Indian stock market history. 1830's Business on corporate stocks and shares in Bank and Cotton presses started in Bombay. 1860-1865 Cotton price bubble as a result of the American Civil War 1870 – 90's Sharp increase in share prices of jute industries followed by a boom in tea stocks and coal 1978-79 Base year of Sensex, defined to be 100. †¦wikipedia. rg/†¦/Bombay_Stock_Exch†¦ 2/6 9/27/2010 Bombay Stock Exchange – Wikipedia, th†¦ 1986 Sensex first compiled[8] using a market Capitalization-Weighted methodology for 30 component stocks representing well-established companies across key sectors. 30 October 2006 The Sensex on October 30, 2006 crossed the magical figure of 13,000 and closed at 13,024. 26 points, up 117. 45 points or 0. 9%. It took 135 days for the Sensex to move from 12,000 to 13,000 and 123 days to move from 12,500 to 13,000. 5 December 2006 The Sensex on December 5, 2006 crossed the 14,000-mark to touch 14,028 points.It t ook 36 days for the Sensex to move from 13,000 to the 14,000 mark. 6 July 2007 The Sensex on July 6, 2007 crossed the magical figure of 15,000 to touch 15,005 points in afternoon trade. It took seven months for the Sensex to move from 14,000 to 15,000 points. 19 September 2007 The Sensex scaled yet another milestone during early morning trade on September 19, 2007. Within minutes after trading began, the Sensex crossed 16,000, rising by 450 points from the previous close. The 30-share Bombay Stock Exchange's sensitive index took 53 days to reach 16,000 from 15,000. Nifty also touched a new high at 4659, up 113 points.The Sensex finally ended with a gain of 654 points at 16,323. The NSE Nifty gained 186 points to close at 4,732. 26 September 2007 The Sensex scaled yet another height during early morning trade on September 26, 2007. Within minutes after trading began, the Sensex crossed the 17,000-mark . Some profit taking towards the end, saw the index slip into red to 16,887  œ down 187 points from the day's high. The Sensex ended with a gain of 22 points at 16,921. 9 October 2007 The BSE Sensex crossed the 18,000-mark on October 9, 2007. It took just 8 days to cross 18,000 points from the 17,000 mark.The index zoomed to a new all-time intra-day high of 18,327. It finally gained 789 points to close at an all-time high of 18,280. The market set several new records including the biggest single day gain of 789 points at close, as well as the largest intra-day gains of 993 points in absolute term backed by frenzied buying after the news of the UPA and Left meeting on October 22 put an end to the worries of an impending election. 15 October 2007 The Sensex crossed the 19,000-mark backed by revival of funds-based buying in blue chip stocks in metal, capital goods and refinery sectors.The index gained the last 1,000 points in just four trading days. The index touched a fresh all-time intra-day high of 19,096, and finally ended with a smart gain of 640 points at 19,059. The Nifty gained 242 points to close at 5,670. 29 October 2007 The Sensex crossed the 20,000 mark on the back of aggressive buying by funds ahead of the US Federal Reserve meeting. The index took only 10 trading days to gain 1,000 points after the index crossed the 19,000-mark on October 15. The major drivers of today's rally were index heavyweights Larsen and Toubro, Reliance Industries, ICICI Bank, HDFC Bank and SBI among others.The 30-share index spurted in the last five minutes of trade to fly-past the crucial level and scaled a new intra-day peak at 20,024. 87 points before ending at its fresh closing high of 19,977. 67, a gain of 734. 50 points. The NSE Nifty rose to a record high 5,922. 50 points before ending at 5,905. 90, showing a hefty gain of 203. 60 points. 8 January 2008 The sensex peaks. It crossed the 21,000 mark in intra-day trading after 49 trading sessions. This was backed by high market confidence of increased FII investment and strong corporate results for the third quarter.However, it later fell back due to profit booking. †¦wikipedia. org/†¦/Bombay_Stock_Exch†¦ 3/6 9/27/2010 Bombay Stock Exchange – Wikipedia, th†¦ 13 June 2008 The sensex closed below 15,200 mark, Indian market suffer with major downfall from January 21, 2008 25 June 2008 The sensex touched an intra day low of 13,731 during the early trades, then pulled back and ended up at 14,220 amidst a negative sentiment generated on the Reserve Bank of India hiking CRR by 50 bps. FII outflow continued in this week. 2 July 2008 The sensex hit an intra day low of 12,822. 0 on July 2, 2008. This is the lowest that it has ever been in the past year. Six months ago, on January 10, 2008, the market had hit an all time high of 21206. 70. This is a bad time for the Indian markets, although Reliance and Infosys continue to lead the way with mostly positive results. Bloomberg lists them as the top two gainers for the Sensex, closely followed by ICICI Bank an d ITC Ltd. 6 October 2008 The sensex closed at 11801. 70 hitting the lowest in the past 2 years. 10 October 2008 The Sensex today closed at 10527,800. 1 points down from the previous day having seen an intraday fall of as large as 1063 points. Thus, this week turned out to be the week with largest percentage fall in the SenseX 18 May 2009 After the result of 15th Indian general election Sensex gained 2100. 79 points from the previous close of 12173. 42, a record one-day gain. In the opening trade itself the Sensex evinced a 15% gain over the previous close which led to a two-hour suspension in trading. After trading resumed, the Sensex surged again, leading to a full day suspension of trading. BSE indicesFor the premier stock exchange that pioneered the securities transaction business in India, over a century of experience is a proud achievement. A lot has changed since 1875 when 318 persons by paying a then princely amount of Re. 1, became members of what today is called Bombay Sto ck Exchange Limited (BSE). Over the decades, the stock market in the country has passed through good and bad periods. The journey in the 20th century has not been an easy one. Till the decade of eighties, there was no measure or scale that could precisely measure the various ups and downs in the Indian stock market.BSE, in 1986, came out with a Stock Index-SENSEX- that subsequently became the barometer of the Indian stock market. The launch of SENSEX in 1986 was later followed up in January 1989 by introduction of BSE National Index (Base: 1983-84 = 100). It comprised 100 stocks listed at five major stock exchanges in India Mumbai, Calcutta, Delhi, Ahmedabad and Madras. The BSE National Bombay Stock Exchange Index was renamed BSE-100 Index from October 14, 1996 and since then, it is being calculated taking into consideration only the prices of stocks listed at BSE.BSE launched the dollar-linked version of BSE-100 index on May 22, 2006. With a view to provide a better representation of the increasing number of listed companies, larger market capitalization and the new industry sectors, BSE launched on 27th May, 1994 two new index series viz. , the ‘BSE200' and the ‘DOLLEX-200'. Since then, BSE has come a long way in attuning itself to the varied needs of †¦wikipedia. org/†¦/Bombay_Stock_Exch†¦ 4/6 9/27/2010 Bombay Stock Exchange – Wikipedia, th†¦ investors and market participants.In order to fulfill the need for still broader, segment-specific and sector-specific indices, BSE has continuously been increasing the range of its indices. BSE-500 Index and 5 sectoral indices were launched in 1999. In 2001, BSE launched BSE-PSU Index, DOLLEX-30 and the country's first free-float based index – the BSE TECk Index. Over the years, BSE shifted all its indices to the free-float methodology (except BSE-PSU index). BSE disseminates information on the Price-Earnings Ratio, the Price to Book Value Ratio and the Dividend Yield Perc entage on day-to-day basis of all its major indices.The values of all BSE indices are updated on real time basis during market hours and displayed through the BOLT system, BSE website and news wire agencies. All BSE Indices are reviewed periodically by the BSE Index Committee. This Committee which comprises eminent independent finance professionals frames the broad policy guidelines for the development and maintenance of all BSE indices. The BSE Index Cell carries out the day-to-day maintenance of all indices and conducts research on development of new indices. [9] Sensex correlation with emerging market indicesSensex is significantly correlated with the stock indices of other emerging markets[10][11] Awards The World Council of Corporate Governance has awarded the Golden Peacock Global CSR Award for BSE's initiatives in Corporate Social Responsibility (CSR). The Annual Reports and Accounts of BSE for the year ended March 31, 2006 and March 31 2007 have been awarded the ICAI awards for excellence in financial reporting. The Human Resource Management at BSE has won the Asia – Pacific HRM awards for its efforts in employer branding through talent management at work, health management at work and excellence in HR through technologySee also Phiroze Jeejeebhoy Towers Clause 49 National Stock Exchange of India Companies listed on the Bombay Stock Exchange List of South Asian stock exchanges References 1. ^ World-exchanges. org (http://www. world-exchanges. org) 2. ^ World-exchanges. org (http://www. world-exchanges. org) 3. ^ â€Å"BSE – Key statistics† (http://www. bseindia. com/about/st_key/list_cap_raised. asp) . Bseindia. com. http://www. bseindia. com/about/st_key/list_cap_raised. asp. Retrieved 2010-08-26. 4. ^ World Federation of Exchanges (2007) (http://www. world-exchanges. rg/publications/EQU1107. pdf) â€Å"World †¦wikipedia. org/†¦/Bombay_Stock_Exch†¦ 5/6 9/27/2010 Bombay Stock Exchange – Wikipedia, th†¦ 5 . 6. 7. 8. 9. 10. 11. Federation of Exchanges (2007)† ^ BSE website, (http://www. bseindia. com/about/st_key/list_cap_raised. asp) â€Å"Listing and Capital Raised† ^ Market Hours, Bombay Stock Exchange via Wikinvest ^ â€Å"BSEIndia† (http://www. bseindia. com/about/tech. asp) . BSEIndia. http://www. bseindia. com/about/tech. asp. Retrieved 2010-07-28. ^ â€Å"BSEIndia† (http://www. bseindia. com/about/abindices/bse30. asp) .BSEIndia. 2003-09-01. http://www. bseindia. com/about/abindices/bse30. asp. Retrieved 2010-07-28. ^ â€Å"BSEIndia† (http://www. bseindia. com/about/abindices/preface. asp) . BSEIndia. http://www. bseindia. com/about/abindices/preface. asp. Retrieved 2010-08-26. ^ â€Å"BSE SENSEX Index Chart – Yahoo! Finance† (http://finance. yahoo. com/echarts? s=%5EBSESN#chart1:symbol=^bsesn;range=my;compare=^gspc+eww+ewy;indicator=volume;charttype=line;cros shair=on;ohlcvalues=0;logscale=on;source=undefined) . Finance. yahoo. com. http://finance. yahoo. com/echarts? =%5EBSESN#chart1:symbol=^bsesn;range=my;compare=^gspc+eww+ewy;indicator=volume;charttype=line;cros shair=on;ohlcvalues=0;logscale=on;source=undefined. Retrieved 2010-07-28. ^ http://www. reutersindia. net/ Asia Technical Analysis with Phil Smith External links Official website (http://www. bseindia. com/) BSE to launch Sensex Futures in US (http://birlaa. com/2008/04/04/bse-to-launch-sensex-futures-trading-inus/) The Evolution Of Indian Stock Market (http://shareskool. com/articles/ViewArticle. inf? article=The+Evolution+Of+Indian+Stock+Market;article_Id=1) Retrieved from â€Å"http://en. ikipedia. org/wiki/Bombay_Stock_Exchange† Categories: Bombay Stock Exchange | Companies established in 1875 | Stock exchanges in India | Stock exchanges in Asia | Companies based in Mumbai This page was last modified on 25 September 2010 at 00:14. Text is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. See Terms of Use for details. Wikipedia ® is a registered trademark of the Wikimedia Foundation, Inc. , a non-profit organization. †¦wikipedia. org/†¦/Bombay_Stock_Exch†¦ 6/6